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Characterisation of pectin and optimization of pectinase enzyme from novel Streptomyces fumigatiscleroticus VIT-SP4 for drug delivery and concrete crack-healing applications: An eco-friendly approach.

ABSTRACT: Pectinases are enzymes which are widely distributed in microbes that are present in pectin enriched sites. The agro-industrial residues can be utilized in the industrial scale for low-cost and efficient pectinase production in an eco-friendly approach. This study employs low-cost substrates (i.e. culinary fruit peels) for maximum pectinase production from novel Streptomyces fumigatiscleroticus VIT-SP4. The extraction and characterization of pectin from different fruit peels were investigated and pectinase activity was analyzed. The orange pectin gave maximum pectinase activity of about 45.93 (U/mL). Further, statistical optimization of process parameters was studied by using Taguchi method showed optimum values of pH-6, temperature -35?°C, orange pectin% - 2.5, incubation time- 48?h and RPM- 200?rpm and pectinase activity was found to be 98.65 (U/mL). The response surface methodology (RSM) was used for the optimization of media components which revealed that starch -1.17%, yeast extract-2%, and orange pectin% - 0.75% produces maximum pectinase of about 170.05 (U/mL). The drug-delivery study showed drug release was not observed at initial pH 3 after 4?h. The immediate drug release was noted at pH 6 caused due to disintegration of pectin by the pectinase activity. The self-healing of cracks by spray culture technique was investigated. The crack healing was observed up to 0.50?mm wide after 12?days. This confirms the ability of actinomycete spores to survive and they react to form calcite complex directly helps in crack healing process. This low-cost microbial pectinase can be used in drug delivery and concrete crack-healing applications sectors in future.

SUBMITTER: Govindaraji PK

PROVIDER: S-EPMC7715466 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature

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Characterisation of pectin and optimization of pectinase enzyme from novel <i>Streptomyces fumigatiscleroticus</i> VIT-SP4 for drug delivery and concrete crack-healing applications: An eco-friendly approach.

Govindaraji Praveen Kumar PK Vuppu Suneetha S

Saudi journal of biological sciences 20200727 12

Pectinases are enzymes which are widely distributed in microbes that are present in pectin enriched sites. The agro-industrial residues can be utilized in the industrial scale for low-cost and efficient pectinase production in an eco-friendly approach. This study employs low-cost substrates (i.e. culinary fruit peels) for maximum pectinase production from novel <i>Streptomyces fumigatiscleroticus</i> VIT-SP4. The extraction and characterization of pectin from different fruit peels were investiga ...[more]

PMID: 33304164

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Project description:In order to reduce carbon dioxide (CO₂) emissions and produce an eco-friendly construction material, a type of concrete that uses a minimal amount of cement, yet still retains equivalent properties to ordinary cement concrete, has been developed and studied all over the world. Hwangtoh, a type of red clay broadly deposited around the world, has traditionally been considered an eco-friendly construction material, with bonus advantages of having health and cost benefits. Presently, Hwangtoh is not commonly used as a modern construction material due to properties such as low strength and high rates of shrinkage cracking. Recent studies, however, have shown that Hwangtoh can be used as a mineral admixture to improve the strength of concrete. In addition, polyethylene terephthalate (PET) fibers recycled from PET bottle waste can be used to control shrinkage cracks in Hwangtoh concrete. Therefore, in this study, performance verification is conducted on newly developed Hwangtoh concrete mixed with short recycled PET fibers. The results show that Hwangtoh concrete has compressive strength, elastic modulus, and pH properties that are similar to these features in ordinary cement concrete. The properties of carbonation depth and creep strain of Hwangtoh concrete, however, are larger and smaller, respectively, than in ordinary cement concrete. According to flexural tests, reinforced concrete (RC) specimens cast with Hwangtoh admixtures (with and without PET fibers) possess similar or better capacities than ordinary RC specimens. The addition of PET fibers significantly improves the structural ductility of RC specimens under normal environmental conditions. However, the implementations of the concrete in aggressive environment must be carefully considered, since a previous study result indicates degradation of its durability performance in aggressive environments, such as seawater [1]. The results of this study validate the possibility of using eco-friendly Hwangtoh concrete reinforced with recycled PET fibers as a structural material for modern construction.

Dataset Information

Characterisation of pectin and optimization of pectinase enzyme from novel Streptomyces fumigatiscleroticus VIT-SP4 for drug delivery and concrete crack-healing applications: An eco-friendly approach.

Publications

Characterisation of pectin and optimization of pectinase enzyme from novel <i>Streptomyces fumigatiscleroticus</i> VIT-SP4 for drug delivery and concrete crack-healing applications: An eco-friendly approach.

Similar Datasets

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