An optimized base editor with efficient C-to-T base editing in zebrafish.

ABSTRACT:

Background

Zebrafish is a model organism widely used for the understanding of gene function, including the fundamental basis of human disease, enabled by the presence in its genome of a high number of orthologs to human genes. CRISPR/Cas9 and next-generation gene-editing techniques using cytidine deaminase fused with Cas9 nickase provide fast and efficient tools able to induce sequence-specific single base mutations in various organisms and have also been used to generate genetically modified zebrafish for modeling pathogenic mutations. However, the editing efficiency in zebrafish of currently available base editors is lower than other model organisms, frequently inducing indel formation, which limits the applicability of these tools and calls for the search of more accurate and ef

SUBMITTER: Zhao Y 

PROVIDER: S-EPMC7716464 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature