Bead-Based Extracellular Vesicle Analysis Using Flow Cytometry.
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ABSTRACT:
SUBMITTER: Yang KS
PROVIDER: S-EPMC7718389 | biostudies-literature | 2020 Dec
REPOSITORIES: biostudies-literature
Publications
Bead-Based Extracellular Vesicle Analysis Using Flow Cytometry.
Yang Katherine S KS Lin Hsing-Ying HY Curley Caleigh C Welch Marisa W MW Wolpin Brian M BM Lee Hakho H Weissleder Ralph R Im Hyungsoon H Castro Cesar M CM
Advanced biosystems 20201025 12
Extracellular vesicles (EVs) represent promising circulating biomarkers for cancers, but their high-throughput analyses in clinical settings prove challenging due to lack of simple, fast, and robust EV assays. Here, a bead-based EV assay detected by flow cytometry is described, which integrates EV capture using microbeads with EV protein analyses by flow cytometry. The assay is fast (<4 h for 48 samples), robust, and compatible with conventional flow cytometry instruments for high-throughput EV ...[more]
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