Dataset Information

Biosynthesis of silver nanoparticles from marine diatoms Chaetoceros sp., Skeletonema sp., Thalassiosira sp., and their antibacterial study.

ABSTRACT: Diatoms are a reservoir of metabolites with diverse applications and silver nanoparticle (AgNP) from diatoms holds immense therapeutic potentials against pathogenic microbes owing to their silica frustules. In the present study, Chaetoceros sp., Skeletonema sp., and Thalassiosira sp were used for synthesis of AgNP. The average particle size of AgNP synthesized was 149.03?±?3.0?nm, 186.73?±?4.9?nm, and 239.46?±?44.3?nm as reported in DLS whereas 148.3?±?46.8?nm, 238.0?±?60.9?nm, and 359.8?±?92.33?nm in SEM respectively. EDX analysis strongly indicates the confirmation of AgNP displaying a sharp peak of Ag⁺ ions within the spectra. High negative zeta potential values indicate a substantial degree of stabilization even after three months. The antibacterial efficacy of biosynthesized AgNP tested against Aeromonas sp., Escherichia coli, Bacillus subtilis, Staphylococcus aureus, and Streptococcus pneumonia exhibits broad-spectrum antibacterial activity. This study encourages the synthesis of diatom based AgNP for a variety of applications owing least toxicity and biodegradable nature.

SUBMITTER: Mishra B

PROVIDER: S-EPMC7721619 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature

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Biosynthesis of silver nanoparticles from marine diatoms Chaetoceros sp., Skeletonema sp., Thalassiosira sp., and their antibacterial study.

Mishra Bharti B Saxena Abhishek A Tiwari Archana A

Biotechnology reports (Amsterdam, Netherlands) 20201125

Diatoms are a reservoir of metabolites with diverse applications and silver nanoparticle (AgNP) from diatoms holds immense therapeutic potentials against pathogenic microbes owing to their silica frustules. In the present study, Chaetoceros sp., Skeletonema sp., and Thalassiosira sp were used for synthesis of AgNP. The average particle size of AgNP synthesized was 149.03 ± 3.0 nm, 186.73 ± 4.9 nm, and 239.46 ± 44.3 nm as reported in DLS whereas 148.3 ± 46.8 nm, 238.0 ± 60.9  ...[more]

PMID: 33312881

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Project description:Purpose:In this study, silver nanoparticles (AgNPs) were biosynthesized using culture supernatant of strain Shewanella sp. ARY1, characterized and their antibacterial activity was investigated against Gram-negative bacteria Escherichia coli and Klebsiella pneumoniae. Methods:The strain Shewanella sp. ARY1 was isolated from river Yamuna, Delhi and used for biosynthesis of AgNPs via extracellular approach. Biosynthesized AgNPs were characterized by UV-Visible (UV-Vis) spectrophotometer, fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), energy dispersive X-ray (EDX), transmission electron microscopy (TEM) and scanning electron microscopy (SEM). Antibacterial activity of AgNPs was determined by well diffusion, broth microdilution and streaking plate assay to determine the zone of inhibition (ZOI), minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC), respectively. The effect of AgNPs on treated bacteria was investigated by electron microscopy analysis. Further, the biocompatibility of AgNPs was tested against mice erythrocytes (RBC) by hemolytic assay. Results:The UV-Vis spectral analysis revealed absorption maxima at 450 nm which confirmed the formation of AgNPs. The FTIR analysis suggested the involvement of various supernatant biomolecules, as reducing and capping agents in the synthesis of AgNPs. The XRD and EDX analysis confirmed the crystalline and metallic nature of AgNPs, respectively. The TEM and SEM analysis showed nanoparticles were spherical with an average size of 38 nm. The biosynthesized AgNPs inhibited the growth and formed a clear zone of inhibition (ZOI) against tested Gram-negative strains. The MIC and MBC were determined as 8-16 µg/mL and 32 µg/mL, respectively. Further, electron microscopy analysis of treated cells showed that AgNPs can damage the outer membrane, release of cytoplasmic contents, and alter the normal morphology of Gram-negative bacteria, leading to cell death. The hemolytic assay indicated that the biosynthesized AgNPs were biocompatible at low dose concentrations. Conclusion:This study demonstrates an eco-friendly process for extracellular synthesis of AgNPs using Shewanella sp. ARY1 and these AgNPs exhibited excellent antibacterial activity, which may be used to combat Gram-negative pathogens.

Dataset Information

Biosynthesis of silver nanoparticles from marine diatoms Chaetoceros sp., Skeletonema sp., Thalassiosira sp., and their antibacterial study.

Publications

Biosynthesis of silver nanoparticles from marine diatoms <i>Chaetoceros</i> sp., <i>Skeletonema</i> sp., <i>Thalassiosira</i> sp., and their antibacterial study.

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