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Author Correction: VEGFC negatively regulates the growth and aggressiveness of medulloblastoma cells.

ABSTRACT: A Correction to this paper has been published: https://doi.org/10.1038/s42003-020-01502-2.

SUBMITTER: Penco-Campillo M

PROVIDER: S-EPMC7721738 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature

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Author Correction: VEGFC negatively regulates the growth and aggressiveness of medulloblastoma cells.

Penco-Campillo Manon M Comoglio Yannick Y Feliz Morel Álvaro Javier ÁJ Hanna Rita R Durivault Jérôme J Leloire Magalie M Mejias Bastien B Pagnuzzi Marina M Morot Amandine A Burel-Vandenbos Fanny F Selby Matthew M Williamson Daniel D Clifford Steven C SC Claren Audrey A Doyen Jérôme J Picco Vincent V Martial Sonia S Pagès Gilles G

Communications biology 20201207 1

A Correction to this paper has been published: https://doi.org/10.1038/s42003-020-01502-2. ...[more]

PMID: 33288846

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Project description:Hypoxic zones are common features of metastatic tumors. Due to inactivation of the von Hippel-Lindau gene (VHL), renal cell carcinomas (RCC) show constitutive stabilization of the alpha subunit of the hypoxia-inducible factor (HIF). Thus, RCC represents a model of chronic hypoxia. Development of the lymphatic network is dependent on vascular endothelial growth factor C (VEGFC) and lies at the front line of metastatic spreading. Here, we addressed the role of VEGFC in RCC aggressiveness and the regulation of its expression in hypoxia. Methods: Transcriptional and post transcriptional regulation of VEGFC expression was evaluated by qPCR and with reporter genes. The involvement of HIF was evaluated using a siRNA approach. Experimental RCC were performed with immuno-competent/deficient mice using human and mouse cells knocked-out for the VEGFC gene by a CRISPR/Cas9 method. The VEGFC axis was analyzed with an online available data base (TCGA) and using an independent cohort of patients. Results: Hypoxia induced VEGFC protein expression but down-regulated VEGFC gene transcription and mRNA stability. Increased proliferation, migration, over-activation of the AKT signaling pathway and enhanced expression of mesenchymal markers characterized VEGFC-/- cells. VEGFC-/- cells did not form tumors in immuno-deficient mice but developed aggressive tumors in immuno-competent mice. These tumors showed down-regulation of markers of activated lymphocytes and M1 macrophages, and up-regulation of M2 macrophages markers and programmed death ligand 1 (PDL1). Over-expression of lymphangiogenic genes including VEGFC was linked to increased disease-free and overall survival in patients with non-metastatic tumors, whereas its over-expression correlated with decreased progression-free and overall survival of metastatic patients. Conclusion: Our study revisited the admitted dogma linking VEGFC to tumor aggressiveness. We conclude that targeting VEGFC for therapy must be considered with caution.

Dataset Information

Author Correction: VEGFC negatively regulates the growth and aggressiveness of medulloblastoma cells.

Publications

Author Correction: VEGFC negatively regulates the growth and aggressiveness of medulloblastoma cells.

Similar Datasets

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