Organic solvent stability and long-term storage of myoglobin-based carbene transfer biocatalysts.

ABSTRACT: Recent years have witnessed a rapid increase in the application of enzymes for chemical synthesis and manufacturing, including the industrial-scale synthesis of pharmaceuticals using multienzyme processes. From an operational standpoint, these bioprocesses often require robust biocatalysts capable of tolerating high concentrations of organic solvents and possessing long shelflife stability. In this work, we investigated the activity and stability of myoglobin (Mb)-based carbene transfer biocatalysts in the presence of organic solvents and after lyophilization. Our studies demonstrate that Mb-based cyclopropanases possess remarkable organic solvent stability, maintaining high levels of activity and stereoselectivity in the presence of up to 30%-50% (v/v) concentrations of various organic so

SUBMITTER: Pineda-Knauseder AJ 

PROVIDER: S-EPMC7722212 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature