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A dual-deaminase CRISPR base editor enables concurrent adenine and cytosine editing.

ABSTRACT: Existing adenine and cytosine base editors induce only a single type of modification, limiting the range of DNA alterations that can be created. Here we describe a CRISPR-Cas9-based synchronous programmable adenine and cytosine editor (SPACE) that can concurrently introduce A-to-G and C-to-T substitutions with minimal RNA off-target edits. SPACE expands the range of possible DNA sequence alterations, broadening the research applications of CRISPR base editors.

SUBMITTER: Grunewald J

PROVIDER: S-EPMC7723518 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature

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A dual-deaminase CRISPR base editor enables concurrent adenine and cytosine editing.

Grünewald Julian J Zhou Ronghao R Lareau Caleb A CA Garcia Sara P SP Iyer Sowmya S Miller Bret R BR Langner Lukas M LM Hsu Jonathan Y JY Aryee Martin J MJ Joung J Keith JK

Nature biotechnology 20200601 7

Existing adenine and cytosine base editors induce only a single type of modification, limiting the range of DNA alterations that can be created. Here we describe a CRISPR-Cas9-based synchronous programmable adenine and cytosine editor (SPACE) that can concurrently introduce A-to-G and C-to-T substitutions with minimal RNA off-target edits. SPACE expands the range of possible DNA sequence alterations, broadening the research applications of CRISPR base editors. ...[more]

PMID: 32483364

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