Unknown

Dataset Information

0

Human Papillomavirus Infections in Cervical Samples From HIV-Positive Women: Evaluation of the Presence of the Nonavalent HPV Genotypes and Genetic Diversity.


ABSTRACT: Non-nonavalent vaccine (9v) Human papillomavirus (HPV) types have been shown to have high prevalence among HIV-positive women. Here, 1444 cervical samples were tested for HPV DNA positivity. Co-infections of the 9v HPV types with other HPV types were evaluated. The HPV81 L1 and L2 genes were used to investigate the genetic variability of antigenic epitopes. HPV-positive samples were genotyped using the HPVCLART2 assay. The L1 and L2 protein sequences were analyzed using a self-optimized prediction method to predict their secondary structure. Co-occurrence probabilities of the 9v HPV types were calculated. Non9v types represented 49% of the HPV infections; 31.2% of the non9v HPV types were among the low-grade squamous intraepithelial lesion samples, and 27.3% among the high-grade squamous intraepithelial lesion samples, and several genotypes were low risk. The co-occurrence of 9v HPV types with the other genotypes was not correlated with the filogenetic distance. HPV81 showed an amino-acid substitution within the BC loop (N75Q) and the FGb loop (T315N). In the L2 protein, all of the mutations were located outside antigenic sites. The weak cross-protection of the 9v types suggests the relevance of a sustainable and effective screening program, which should be implemented by HPV DNA testing that does not include only high-risk types.

SUBMITTER: Sias C 

PROVIDER: S-EPMC7723855 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

altmetric image

Publications

Human Papillomavirus Infections in Cervical Samples From HIV-Positive Women: Evaluation of the Presence of the Nonavalent HPV Genotypes and Genetic Diversity.

Sias Catia C   Guarrasi Valerio V   Minosse Claudia C   Lapa Daniele D   Del Nonno Franca F   Capobianchi Maria Rosaria MR   Garbuglia Anna Rosa AR   Del Porto Paola P   Paci Paola P  

Frontiers in microbiology 20201125


Non-nonavalent vaccine (9v) Human papillomavirus (HPV) types have been shown to have high prevalence among HIV-positive women. Here, 1444 cervical samples were tested for HPV DNA positivity. Co-infections of the 9v HPV types with other HPV types were evaluated. The HPV81 <i>L1</i> and <i>L2</i> genes were used to investigate the genetic variability of antigenic epitopes. HPV-positive samples were genotyped using the HPVCLART2 assay. The L1 and L2 protein sequences were analyzed using a self-opti  ...[more]

Similar Datasets

| S-EPMC4978368 | biostudies-literature
| S-EPMC4975475 | biostudies-literature
| S-EPMC5637711 | biostudies-literature
| S-EPMC5932258 | biostudies-literature
| S-EPMC7982446 | biostudies-literature
| S-EPMC2720239 | biostudies-other
| S-EPMC4166201 | biostudies-literature
| S-EPMC6834066 | biostudies-literature
| S-EPMC5399941 | biostudies-literature
| S-EPMC7003740 | biostudies-literature