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Plasma circular RNA panel acts as a novel diagnostic biomarker for colorectal cancer detection.

ABSTRACT: Circular RNAs (circRNAs) can function as key regulators of oncogenic processes, making them ideal diagnostic biomarkers of many cancers. However, few studies to date have reported on plasma circRNA profiles associated with colorectal cancer (CRC). To that end, we herein employed microarray- and qRT-PCR-based approaches to evaluate circulating plasma circRNAs in CRC patients. Area under the receiver operating characteristic curve (AUC) values were then used to assess the diagnostic utility of these circRNAs. We ultimately determined that hsa_circ_0001900, hsa_circ_0001178, and hsa_circ_0005927 were upregulated in the plasma of CRC patients relative to healthy controls and were correlated with clinicopathological findings in these patients. We further established that a panel composed of these three circRNAs (CircPanel) was able to differentiate between patients with and without CRC more reliably than CEA (carcinoembryonic antigen) (AUC, 0.859 [95% confidence interval, CI: 0.805-0.903] vs. 0.698 [0.631-0.759], P=0.0003), enabling us to detect patients with CEA-negative CRC. In conclusion, our study reveals that CircPanel could serve as a promising potential biomarker for CRC diagnosis.

SUBMITTER: Li J

PROVIDER: S-EPMC7724351 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Plasma circular RNA panel acts as a novel diagnostic biomarker for colorectal cancer detection.

Li Jipeng J Song Yulan Y Wang Jianhua J Huang Jian J

American journal of translational research 20201115 11

Circular RNAs (circRNAs) can function as key regulators of oncogenic processes, making them ideal diagnostic biomarkers of many cancers. However, few studies to date have reported on plasma circRNA profiles associated with colorectal cancer (CRC). To that end, we herein employed microarray- and qRT-PCR-based approaches to evaluate circulating plasma circRNAs in CRC patients. Area under the receiver operating characteristic curve (AUC) values were then used to assess the diagnostic utility of the ...[more]

PMID: 33312376

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Project description:Background: The morbidity and mortality of gastric cancer (GC) remain high worldwide. With the advent of the Human Genome Sequencing Project, circular RNAs (circRNAs) have attracted widespread attention in cancer research due to their stable ring structure. Our aim was to identify differentially expressed circRNAs in GC and explore their potential roles in GC diagnosis, treatment, and prognostic prediction. Methods: Large-scale gene screening was performed in three pairs of GC tissues and adjacent noncancerous tissues using high-throughput sequencing. The expression of hsa_circ_0001821 was detected in 80 pairs of tissue samples by quantitative real-time PCR (qRT-PCR). Stability of the ring structure of hsa_circ_0001821 RNA was verified by exonuclease digestion assay, and its diagnostic value was evaluated by receiver operating characteristic (ROC) analysis. In addition, the location of hsa_circ_0001821 in GC cells was detected by nucleoplasm separation assay. Results: A total of 25,303 circRNAs were identified, among which 2,007 circRNAs were differentially expressed (fold change > 2.0, P < 0.05). Further validation disclosed that hsa_circ_0001821 was significantly downregulated in the 80 pairs of GC tissues and 30 whole-blood specimens obtained from the GC patients. The specificity of hsa_circ_0001821 in GC was higher than that in other solid tumors. In addition, hsa_circ_0001821 was relatively stable after RNA exonuclease digestion. Clinicopathological parameter analysis showed that hsa_circ_0001821 was negatively correlated with tumor depth (r = -0.255, P = 0.022) and lymph node metastasis (r = -0.235, P = 0.036). Area under the curve (AUC) analysis showed that the diagnostic efficiency of circulating hsa_circ_0001821 in distinguishing GC patients was higher than that in GC tissues (0.872, 95%CI: 0.767-0.977 vs. 0.792, 95%CI: 0.723-0.861). Combined use of circulating hsa_circ_0001821 with the existing tumor markers yielded the largest AUC of 0.933. Finally, hsa_circ_0001821 was demonstrated to mainly locate in the cytoplasm, implying that it played a potential regulatory role in GC at the posttranscriptional level. Conclusion: Hsa_circ_0001821 may prove to be a new and promising potential biomarker for GC diagnosis.

Dataset Information

Plasma circular RNA panel acts as a novel diagnostic biomarker for colorectal cancer detection.

Publications

Plasma circular RNA panel acts as a novel diagnostic biomarker for colorectal cancer detection.

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