Dataset Information

Optimum Operating Frequency for Electrocoalescence Induced by Pulsed Corona Discharge.

ABSTRACT: Current oil-water separation methods require a significant power, a high processing time, and costly equipment, which typically yield low treatment efficiency. Pulsed direct current (dc) electric fields and recently nonuniform electric fields caught considerable attention in the petroleum industry research in order to address the most common oil-water separation issues such as chain formation, partial coalescence, and low efficiency in either the energy consumption or coalescence rate. Here, a contact-less charge injection method induced by corona discharge is utilized to investigate the impacts of nonuniform and pulsed dc electric fields on the coalescence of water droplets inside an oil medium. The operating process parameters were experimentally calibrated and optimized with the goal of increasing the effectiveness and energy consumption efficiency of the coalescence process. High-speed imaging and image processing techniques were used in order to investigate the effect of different active forces (i.e., dipole-dipole interaction, migratory coalescence, or electrophoresis, and dielectrophoresis) during the coalescence process. Different pulsed dc frequencies and pure dc waveforms were utilized and their impact on the coalescence of water droplets was investigated. An optimal coalescence recipe was proposed by continuous measurement of the distance, velocity, and acceleration of the coalescing water droplets. The results of this study suggest use of pulsed dc and pure dc electric fields for coalescence of water droplets in concentrated and dispersed emulsions, respectively.

SUBMITTER: Shahbaznezhad M

PROVIDER: S-EPMC7726783 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature

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Optimum Operating Frequency for Electrocoalescence Induced by Pulsed Corona Discharge.

Shahbaznezhad Mohcen M Dehghanghadikolaei Amir A Sojoudi Hossein H

ACS omega 20201119 48

Current oil-water separation methods require a significant power, a high processing time, and costly equipment, which typically yield low treatment efficiency. Pulsed direct current (dc) electric fields and recently nonuniform electric fields caught considerable attention in the petroleum industry research in order to address the most common oil-water separation issues such as chain formation, partial coalescence, and low efficiency in either the energy consumption or coalescence rate. Here, a c ...[more]

PMID: 33324808

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Project description:Soil-borne pathogenic microorganisms are known to cause extensive crop losses. Agrobacterium tumefaciens, a member of the Proteobacteria, causes the neoplastic crown gall disease in plants. Plant protection is mainly based on toxic chemicals that are harmful to the environment. The use of cold atmospheric-pressure plasma is an attractive method for microbial eradication. Its antimicrobial mechanism includes the formation of large quantities of reactive oxygen species (ROS). The advantages of eradicating bacteria using cold plasma are not needed for chemicals, short treatment, and environmental temperatures. This study examined the impact of plasma corona discharge exposure on A. tumefaciens viability, membrane permeability, relative cell size, and ROS formation. The results showed that 90 s of plasma exposure led to a reduction by four orders of magnitude when the initial concentration was 1 × 107 CFU/mL and in a dry environment. When the initial concentration was 1 × 106 CFU/mL, 45 s of exposure resulted in total bacterial eradication. In a liquid environment, in an initial concentration of 2.02 × 106 CFU/mL, there was no complete bacterial eradication even at the most prolonged examined exposure (90 s). The influence of plasma treatment on the membrane permeability of A. tumefaciens, and their possible recovery, were analyzed using flow cytometer analysis using propidium iodide (PI). When the plasma-treated bacteria were suspended in Luria-Bertani (LB) (rich medium), the PI-positive count of the plasma-treated bacteria after two hours was 12 ± 3.9%. At the 24th hour, this percentage was only 1.74 ± 0.6%, as the control (0.7 ± 0.1%). These results may indicate the repair of the plasma-treated bacteria that were suspended in LB. At the 24th hour, the relative cell size of the treated bacteria shifted to the right, to ~3 × 104 forward side scatter (FSC), about 0.5-fold higher than the untreated cells. Measurement of the ROS showed that the intracellular fluorescence of the 90-s plasma-treated cells led to significant fluorescence formation of 32 relative fluorescence units (RFU)/cell (9 × 104 fold, compared to the nontreated cells). This study showed that cold plasma is a useful method for A. tumefaciens eradication. The eradication mechanism involves ROS generation, membrane permeability, and changes in cell size.

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Optimum Operating Frequency for Electrocoalescence Induced by Pulsed Corona Discharge.

Publications

Optimum Operating Frequency for Electrocoalescence Induced by Pulsed Corona Discharge.

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