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Sugar transport for enhanced xylose utilization in Ashbya gossypii.


ABSTRACT: The co-utilization of mixed (pentose/hexose) sugars constitutes a challenge for microbial fermentations. The fungus Ashbya gossypii, which is currently exploited for the industrial production of riboflavin, has been presented as an efficient biocatalyst for the production of biolipids using xylose-rich substrates. However, the utilization of xylose in A. gossypii is hindered by hexose sugars. Three A. gossypii homologs (AFL204C, AFL205C and AFL207C) of the yeast HXT genes that code for hexose transporters have been identified and characterized by gene-targeting approaches. Significant differences in the expression profile of the HXT homologs were found in response to different concentrations of sugars. More importantly, an amino acid replacement (N355V) in AFL205Cp, introduced by CRISPR/Cas9-mediated genomic edition, notably enhanced the utilization of xylose in the presence of glucose. Hence, the introduction of the afl205c-N355V allele in engineered strains of A. gossypii will further benefit the utilization of mixed sugars in this fungus.

SUBMITTER: Diaz-Fernandez D 

PROVIDER: S-EPMC7728639 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature

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Sugar transport for enhanced xylose utilization in Ashbya gossypii.

Díaz-Fernández David D   Muñoz-Fernández Gloria G   Martín Victoria Isabel VI   Revuelta José Luis JL   Jiménez Alberto A  

Journal of industrial microbiology & biotechnology 20201009 12


The co-utilization of mixed (pentose/hexose) sugars constitutes a challenge for microbial fermentations. The fungus Ashbya gossypii, which is currently exploited for the industrial production of riboflavin, has been presented as an efficient biocatalyst for the production of biolipids using xylose-rich substrates. However, the utilization of xylose in A. gossypii is hindered by hexose sugars. Three A. gossypii homologs (AFL204C, AFL205C and AFL207C) of the yeast HXT genes that code for hexose tr  ...[more]

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