Dataset Information

CD8⁺ T cell gene expression analysis identifies differentially expressed genes between multiple sclerosis patients and healthy controls.

ABSTRACT:

Background

Genetic and clinical observations have indicated T cells are involved in MS pathology. There is little insight in how T cells are involved and whether or not these can be used as markers for MS.

Objectives

Analysis of the gene expression profiles of circulating CD8⁺ T cells of MS patients compared to healthy controls.

Methods

RNA from purified CD8⁺ T cells was sequenced and analyzed for differential gene expression. Pathway analyses of genes at several p-value cutoffs were performed to identify putative pathways involved.

Results

We identified 36 genes with significant differential gene expression in MS patients. Four genes reached at least 2-fold differences in expression. The majority of differentially expressed genes was higher expressed in MS patients. Genes associated to MS in GWAS showed enrichment amongst the differentially expressed genes. We did not identify enrichment of specific pathways amongst the differentially expressed genes in MS patients.

Conclusions

CD8⁺ T cells of MS patients show differential gene expression, with predominantly higher activity of genes in MS patients. We do not identify specific biological pathways in our study. More detailed analysis of CD8⁺ T cells and subtypes of these may increase understanding of how T cells are involved in MS.

SUBMITTER: Brorson IS

PROVIDER: S-EPMC7731718 | biostudies-literature | 2020 Oct-Dec

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

CD8<sup>+</sup> T cell gene expression analysis identifies differentially expressed genes between multiple sclerosis patients and healthy controls.

Brorson I S IS Eriksson A M AM Leikfoss I S IS Vitelli V V Celius E G EG Lüders T T Berge T T Harbo H F HF Nilsen H H Bos S D SD

Multiple sclerosis journal - experimental, translational and clinical 20201001 4

<h4>Background</h4>Genetic and clinical observations have indicated T cells are involved in MS pathology. There is little insight in how T cells are involved and whether or not these can be used as markers for MS.<h4>Objectives</h4>Analysis of the gene expression profiles of circulating CD8<sup>+</sup> T cells of MS patients compared to healthy controls.<h4>Methods</h4>RNA from purified CD8<sup>+</sup> T cells was sequenced and analyzed for differential gene expression. Pathway analyses of genes ...[more]

PMID: 33343920

Similar Datasets

Project description:BackgroundSecretoglobin 1A1 (SCGB 1A1), also called Clara cell secretory protein, is the most abundantly secreted protein of the airway. The SCGB1A1 gene has been characterized in mammals as a single copy in the genome. However, analysis of the equine genome suggested that horses might have multiple SCGB1A1 gene copies. Non-ciliated lung epithelial cells produce SCGB 1A1 during inhalation of noxious substances to counter airway inflammation. Airway fluid and lung tissue of horses with recurrent airway obstruction (RAO), a chronic inflammatory lung disease affecting mature horses similar to environmentally induced asthma of humans, have reduced total SCGB 1A1 concentration. Herein, we investigated whether horses have distinct expressed SCGB1A1 genes; whether the transcripts are differentially expressed in tissues and in inflammatory lung disease; and whether there is cell specific protein expression in tissues.ResultsWe identified three SCGB1A1 gene copies on equine chromosome 12, contained within a 512-kilobase region. Bioinformatic analysis showed that SCGB1A1 genes differ from each other by 8 to 10 nucleotides, and that they code for different proteins. Transcripts were detected for SCGB1A1 and SCGB1A1A, but not for SCGB1A1P. The SCGB1A1P gene had most inter-individual variability and contained a non-sense mutation in many animals, suggesting that SCGB1A1P has evolved into a pseudogene. Analysis of SCGB1A1 and SCGB1A1A sequences by endpoint-limiting dilution PCR identified a consistent difference affecting 3 bp within exon 2, which served as a gene-specific "signature". Assessment of gene- and organ-specific expression by semiquantitative RT-PCR of 33 tissues showed strong expression of SCGB1A1 and SCGB1A1A in lung, uterus, Fallopian tube and mammary gland, which correlated with detection of SCGB 1A1 protein by immunohistochemistry. Significantly altered expression of the ratio of SCGB1A1A to SCGB1A1 was detected in RAO-affected animals compared to controls, suggesting different roles for SCGB 1A1 and SCGB 1A1A in this inflammatory condition.ConclusionsThis is the first report of three SCGB1A1 genes in a mammal. The two expressed genes code for proteins predicted to differ in function. Alterations in the gene expression ratio in RAO suggest cell and tissue specific regulation and functions. These findings may be important for understanding of lung and reproductive conditions.

Dataset Information

CD8⁺ T cell gene expression analysis identifies differentially expressed genes between multiple sclerosis patients and healthy controls.

Background

Objectives

Methods

Results

Conclusions

Publications

CD8<sup>+</sup> T cell gene expression analysis identifies differentially expressed genes between multiple sclerosis patients and healthy controls.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Dataset Information

CD8+ T cell gene expression analysis identifies differentially expressed genes between multiple sclerosis patients and healthy controls.

Background

Objectives

Methods

Results

Conclusions

Publications

CD8<sup>+</sup> T cell gene expression analysis identifies differentially expressed genes between multiple sclerosis patients and healthy controls.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

CD8⁺ T cell gene expression analysis identifies differentially expressed genes between multiple sclerosis patients and healthy controls.