Dataset Information

Analysis of the lateral slope's impact on the calculation of water-filled rut depth.

ABSTRACT: Accurate calculation of the water-filled rut depth is critical for assessing hydroplaning potential. Nevertheless, due to the difficulty in collecting and calculating the water-filled rut depth, most transportation agencies do not use i, especially in the case of lateral slopes, although water-filled rut depth is a key parameter that impacts driving safety. Contributions of this paper are development of a methodology to reliably compute the water-filled rut depth and quantitatively evaluate the influence of lateral slope on the water-filled rut depth. The proposed method include: 1) acquisition of the high-resolution 3D point cloud data of rut, 2) smooth processing of rut profile through moving average method with Matlab programming, 3) water-filled rut depth computation at different lateral slopes with the assistance of key points based on rut sections. With the variation of water-filled rut depth (?WD), its change rate (?WD), and the calculation error between the rut depth and the water-filled rut depth (?n) as evaluation indexes, the variation law of water-filled rut depth under different lateral slopes is analyzed when considering the severity level and rut shape of the rut profile. Results show that: 1) the increase in lateral slope leads to the reduction of water-filled rut depth; 2) the water-filled rut depth is affected by the rut shape, including rut side wall's slope and the key points' elevation. The accurate calculation of the water-filled rut depth can provide reliable suggestions for safe driving.

SUBMITTER: Yan J

PROVIDER: S-EPMC7732103 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Analysis of the lateral slope's impact on the calculation of water-filled rut depth.

Yan Jiao J Zhang Hongwei H Hui Bing B

PloS one 20201211 12

Accurate calculation of the water-filled rut depth is critical for assessing hydroplaning potential. Nevertheless, due to the difficulty in collecting and calculating the water-filled rut depth, most transportation agencies do not use i, especially in the case of lateral slopes, although water-filled rut depth is a key parameter that impacts driving safety. Contributions of this paper are development of a methodology to reliably compute the water-filled rut depth and quantitatively evaluate the ...[more]

PMID: 33306737

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Project description:BackgroundKnowledge with respect to regulatory systems for cellulase production is prerequisite for exploitation of such regulatory networks to increase cellulase production, improve fermentation efficiency and reduce the relevant production cost. The target of rapamycin (TOR) signaling pathway is considered as a central signaling hub coordinating eukaryotic cell growth and metabolism with environmental inputs. However, how and to what extent the TOR signaling pathway and rapamycin are involved in cellulase production remain elusive.ResultAt the early fermentation stage, high-dose rapamycin (100 μM) caused a temporary inhibition effect on cellulase production, cell growth and sporulation of Trichoderma reesei RUT-C30 independently of the carbon sources, and specifically caused a tentative morphology defect in RUT-C30 grown on cellulose. On the contrary, the lipid content of T. reesei RUT-C30 was not affected by rapamycin. Accordingly, the transcriptional levels of genes involved in the cellulase production were downregulated notably with the addition of rapamycin. Although the mRNA levels of the putative rapamycin receptor trFKBP12 was upregulated significantly by rapamycin, gene trTOR (the downstream effector of the rapamycin-FKBP12 complex) and genes associated with the TOR signaling pathways were not changed markedly. With the deletion of gene trFKBP12, there is no impact of rapamycin on cellulase production, indicating that trFKBP12 mediates the observed temporary inhibition effect of rapamycin.ConclusionOur study shows for the first time that only high-concentration rapamycin induced a transient impact on T. reesei RUT-C30 at its early cultivation stage, demonstrating T. reesei RUT-C30 is highly resistant to rapamycin, probably due to that trTOR and its related signaling pathways were not that sensitive to rapamycin. This temporary influence of rapamycin was facilitated by gene trFKBP12. These findings add to our knowledge on the roles of rapamycin and the TOR signaling pathways play in T. reesei.

Dataset Information

Analysis of the lateral slope's impact on the calculation of water-filled rut depth.

Publications

Analysis of the lateral slope's impact on the calculation of water-filled rut depth.

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