Dataset Information

Host Genetics Background Influence in the Intragastric Trypanosoma cruzi Infection.

ABSTRACT:

Background

Considering the complexity of the factors involved in the immunopathology of Chagas disease, which influence the Chagas' disease pathogenesis, anti-T. cruzi immune response, and chemotherapy outcome, further studies are needed to improve our understanding about these relationships. On this way, in this article we analyzed the host genetic influence on hematological, histopathological and immunological aspects after T. cruzi infection.

Methods

BALB/c and A mice were intragastrically infected with T. cruzi SC2005 strain, isolated from a patient of an outbreak of Chagas disease. Parameters such as parasite load, survival rates, cytokines production, macrophages, T and B cell frequencies, and histopathology analysis were carried out.

Results

BALB/c mice presented higher parasitemia and mortality rates than A mice. Both mouse lineages exhibited hematological alterations suggestive of microcytic hypochromic anemia and histopathological alterations in stomach, heart and liver. The increase of CD8⁺ T cells, in heart, liver and blood, and the increase of CD19⁺ B cells, in liver, associated with a high level of proinflammatory cytokines (IL-6, TNF-?, IFN-?), confer a resistance profile to the host. Although BALB/c animals exhibited the same findings observed in A mice, the response to infection occurred later, after a considerable parasitemia increase. By developing an early response to the infection, A mice were found to be less susceptible to T. cruzi SC2005 infection.

Conclusions

Host genetics background shaping the response to infection. The early development of a cytotoxic cellular response profile with the production of proinflammatory cytokines is important to lead a less severe manifestation of Chagas disease.

SUBMITTER: Domingues CS

PROVIDER: S-EPMC7732431 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Publications

Host Genetics Background Influence in the Intragastric <i>Trypanosoma cruzi</i> Infection.

Domingues Carolina Salles CS Cardoso Flávia de Oliveira FO Hardoim Daiana de Jesus DJ Pelajo-Machado Marcelo M Bertho Alvaro Luiz AL Calabrese Kátia da Silva KDS

Frontiers in immunology 20201124

<h4>Background</h4>Considering the complexity of the factors involved in the immunopathology of Chagas disease, which influence the Chagas' disease pathogenesis, anti-<i>T. cruzi</i> immune response, and chemotherapy outcome, further studies are needed to improve our understanding about these relationships. On this way, in this article we analyzed the host genetic influence on hematological, histopathological and immunological aspects after <i>T. cruzi</i> infection.<h4>Methods</h4>BALB/c and A ...[more]

PMID: 33329529

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