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IGLR-2, a Leucine-Rich Repeat Domain Containing Protein, Is Required for the Host Defense in Caenorhabditis elegans.

ABSTRACT: Enterohemorrhagic Escherichia coli (EHEC), a human pathogen, also infects Caenorhabditis elegans. We demonstrated previously that C. elegans activates the p38 MAPK innate immune pathway to defend against EHEC infection. However, whether a C. elegans pattern recognition receptor (PRR) exists to regulate the immune pathway remains unknown. PRRs identified in other metazoans contain several conserved domains, including the leucine-rich repeat (LRR). By screening a focused RNAi library, we identified the IGLR-2, a transmembrane protein containing the LRR domain, as a potential immune regulator in C. elegans. Our data showed that iglr-2 regulates the host susceptibility to EHEC infection. Moreover, iglr-2 is required for pathogen avoidance to EHEC. The iglr-2 overexpressed strain, which was more resistant to EHEC originally, showed hypersusceptibility to EHEC upon knockdown of the p38 MAPK pathway. Together, our data suggested that iglr-2 plays an important role in C. elegans to defend EHEC by regulating pathogen-avoidance behavior and the p38 MAPK pathway.

SUBMITTER: Kuo CJ 

PROVIDER: S-EPMC7734252 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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IGLR-2, a Leucine-Rich Repeat Domain Containing Protein, Is Required for the Host Defense in <i>Caenorhabditis elegans</i>.

Kuo Cheng-Ju CJ   Hsu Ya-Chu YC   Wang Sin-Tian ST   Liou Bang-Yu BY   Lim Serene Boon-Yuean SB   Chen Yi-Wei YW   Chen Chang-Shi CS  

Frontiers in immunology 20201130

Enterohemorrhagic <i>Escherichia coli</i> (EHEC), a human pathogen, also infects <i>Caenorhabditis elegans</i>. We demonstrated previously that <i>C. elegans</i> activates the p38 MAPK innate immune pathway to defend against EHEC infection. However, whether a <i>C. elegans</i> pattern recognition receptor (PRR) exists to regulate the immune pathway remains unknown. PRRs identified in other metazoans contain several conserved domains, including the leucine-rich repeat (LRR). By screening a focuse  ...[more]

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