Dataset Information

Frequent Association of Colletotrichum Species with Citrus Fruit and Leaf Spot Disease Symptoms and their Genetic Diversity in Ethiopia.

ABSTRACT: Citrus leaf and fruit spot is one of the most important biotic constraints of citrus production in Ethiopia. The symptomatic leaf and fruit samples were collected from 29 orchards of 15 major citrus growing districts of Ethiopia. One hundred sixty-seven fungal isolates were recovered and identified to species level through DNA barcoding; and their relationships were established using multigene phylogeny. The internal transcribed spacers, long subunit and actin gene sequences revealed that those 167 isolates belonged to either Collectotrichum gloeosporioides or Collectotrichum boninense species complexes (sensu lato), but no recovery of Pseudocercospora angolensis, the primary causal agent of the citrus leaf and fruit spot disease. Detached leaf assays confirmed pathogenicity of isolates of both C. gloeosporioides and C. boninense species complexes on citrus. They reproduced disease symptoms and the pathogens were re-isolated from symptomatic tissues. This study reports frequent association of C. gloeosporioides and C. boninense species complexes with citrus fruit and leaf spot disease in Ethiopia. This finding suggests the need for in-depth studies to determine the roles of C. gloeosporioides and C. boninense species complexes in citrus fruit and leaf spot disease epidemiology.

SUBMITTER: Ad M

PROVIDER: S-EPMC7734371 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

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Frequent Association of Colletotrichum Species with Citrus Fruit and Leaf Spot Disease Symptoms and their Genetic Diversity in Ethiopia.

Ad Moges M D Belew B B Admassu A M Yesuf Y S Maina M Sr Ghimire G

Journal of plant pathology & microbiology 20171026 10

Citrus leaf and fruit spot is one of the most important biotic constraints of citrus production in Ethiopia. The symptomatic leaf and fruit samples were collected from 29 orchards of 15 major citrus growing districts of Ethiopia. One hundred sixty-seven fungal isolates were recovered and identified to species level through DNA barcoding; and their relationships were established using multigene phylogeny. The internal transcribed spacers, long subunit and actin gene sequences revealed that those ...[more]

PMID: 33384880

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Project description:Glomerella leaf spot (GLS), caused by Colletotrichum fructicola, is a rapidly emerging disease leading to defoliation, fruit spot, and storage fruit rot on apple in China. Little is known about the mechanisms of GLS pathogenesis. Early transcriptome analysis revealed that expression of the zinc finger transcription factor Ste12 gene in C. fructicola (CfSte12) was upregulated in appressoria and leaf infection. To investigate functions of CfSte12 during pathogenesis, we constructed gene deletion mutants (ΔCfSte12) by homologous recombination. Phenotypic analysis revealed that CfSte12 was involved in pathogenesis of nonwounded apple fruit and leaf, as well as wounded apple fruit. Subsequent histological studies revealed that loss of pathogenicity by ΔCfSte12 on apple leaf was expressed as defects of conidium germination, appressorium development, and appressorium-mediated penetration. Further RNA sequencing-based transcriptome comparison revealed that CfSte12 modulates the expression of genes related to appressorium function (e.g., genes for the tetraspanin PLS1, Gas1-like proteins, cutinases, and melanin biosynthesis) and candidate effectors likely involved in plant interaction. In sum, our results demonstrated that CfSte12 is a key regulator of early apple GLS pathogenesis in C. fructicola In addition, CfSte12 is also needed for sexual development of perithecia and ascospores.IMPORTANCE Glomerella leaf spot (GLS) is an emerging fungal disease of apple that causes huge economic losses in Asia, North America, and South America. The damage inflicted by GLS manifests in rapid necrosis of leaves, severe defoliation, and necrotic spot on the fruit surface. However, few studies have addressed mechanisms of GLS pathogenesis. In this study, we identified and characterized a key pathogenicity-related transcription factor, CfSte12, of Colletotrichum fructicola that contributes to GLS pathogenesis. We provide evidence that the CfSte12 protein regulates many important pathogenic processes of GLS, including conidium germination, appressorium formation, appressorium-mediated penetration, and colonization. CfSte12 also impacts development of structures needed for sexual reproduction which are vital for the GLS disease cycle. These results reveal a key pathogenicity-related transcription factor, CfSte12, in C. fructicola that causes GLS.

Dataset Information

Frequent Association of Colletotrichum Species with Citrus Fruit and Leaf Spot Disease Symptoms and their Genetic Diversity in Ethiopia.

Publications

Frequent Association of Colletotrichum Species with Citrus Fruit and Leaf Spot Disease Symptoms and their Genetic Diversity in Ethiopia.

Similar Datasets

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