Cannabinoid receptor subtype influence on neuritogenesis in human SH-SY5Y cells.

ABSTRACT: Human SH-SY5Y neuroblastoma cells stably expressing exogenous CB₁ (CB₁XS) or CB₂ (CB₂XS) receptors were developed to investigate endocannabinoid signaling in the extension of neuronal projections. Expression of cannabinoid receptors did not alter proliferation rate, viability, or apoptosis relative to parental SH-SY5Y. Transcripts for endogenous cannabinoid system enzymes (diacylglycerol lipase, monoacylglycerol lipase, α/β-hydrolase domain containing proteins 6 and 12, N-acyl phosphatidylethanolamine-phospholipase D, and fatty acid amide hydrolase) were not altered by CB₁ or CB₂ expression. Endocannabinoid ligands 2-arachidonoylglycerol (2-AG) and anandamide were quantitated in SH-SY5Y cells, and diacylglycerol lipase inhib

SUBMITTER: Lyons EL 

PROVIDER: S-EPMC7736467 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature