Ontology highlight
ABSTRACT:
SUBMITTER: Song C
PROVIDER: S-EPMC7736807 | biostudies-literature | 2020 Oct
REPOSITORIES: biostudies-literature
Nucleic acids research 20201201 22
Biosynthesis reprograming is an important way to diversify chemical structures. The large repetitive DNA sequences existing in polyketide synthase genes make seamless DNA manipulation of the polyketide biosynthetic gene clusters extremely challenging. In this study, to replace the ethyl group attached to the C-21 of the macrolide insecticide spinosad with a butenyl group by refactoring the 79-kb gene cluster, we developed a RedEx method by combining Redαβ mediated linear-circular homologous reco ...[more]