Ontology highlight
ABSTRACT:
SUBMITTER: Bell NAW
PROVIDER: S-EPMC7737957 | biostudies-literature | 2020
REPOSITORIES: biostudies-literature
Bell Nicholas A W NAW Molloy Justin E JE
PloS one 20201215 12
We present a fast, inexpensive and robust technique for constructing thin, optically transparent flow-cells with pump-free flow control. Using layers of glass, patterned adhesive tape and polydimethylsiloxane (PDMS) connections, we demonstrate the fabrication of planar devices with chamber height as low as 25 μm and with millimetre-scale (x,y) dimensions for wide-field microscope observation. The method relies on simple benchtop equipment and does not require microfabrication facilities, glass d ...[more]