Ontology highlight
ABSTRACT:
SUBMITTER: Hanami T
PROVIDER: S-EPMC7737986 | biostudies-literature | 2020
REPOSITORIES: biostudies-literature
Hanami Takeshi T Tanabe Tetsuya T Hanashi Takuya T Yamaguchi Mitsushiro M Nakata Hidetaka H Mitani Yasumasa Y Kimura Yasumasa Y Soma Takahiro T Usui Kengo K Isobe Michiko M Ogawa Takashi T Itoh Masayoshi M Hayashizaki Yoshihide Y Kondo Seiji S
PloS one 20201215 12
Here, we report a rapid and ultra-sensitive detection technique for fluorescent molecules called scanning single molecular counting (SSMC). The method uses a fluorescence-based digital measurement system to count single molecules in a solution. In this technique, noise is reduced by conforming the signal shape to the intensity distribution of the excitation light via a circular scan of the confocal region. This simple technique allows the fluorescent molecules to freely diffuse into the solution ...[more]