Human kidney clonal proliferation disclose lineage-restricted precursor characteristics.

ABSTRACT: In-vivo single cell clonal analysis in the adult mouse kidney has previously shown lineage-restricted clonal proliferation within varying nephron segments as a mechanism responsible for cell replacement and local regeneration. To analyze ex-vivo clonal growth, we now preformed limiting dilution to generate genuine clonal cultures from one single human renal epithelial cell, which can give rise to up to 3.4 * 10⁶ cells, and analyzed their characteristics using transcriptomics. A comparison between clonal cultures revealed restriction to either proximal or distal kidney sub-lineages with distinct cellular and molecular characteristics; rapidly amplifying de-differentiated clones and a stably proliferating cuboidal epithelial-appearing clones, respectively. Furthermore, each showed

SUBMITTER: Cohen-Zontag O 

PROVIDER: S-EPMC7745030 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature