Inferring time-dependent population growth rates in cell cultures undergoing adaptation.

ABSTRACT:

Background

The population growth rate is an important characteristic of any cell culture. During sustained experiments, the growth rate may vary due to competition or adaptation. For instance, in presence of a toxin or a drug, an increasing growth rate indicates that the cells adapt and become resistant. Consequently, time-dependent growth rates are fundamental to follow on the adaptation of cells to a changing evolutionary landscape. However, as there are no tools to calculate the time-dependent growth rate directly by cell counting, it is common to use only end point measurements of growth rather than tracking the growth rate continuously.

Results

We present a computer program for inferring the growth rate over time in suspension cells using nothing but cell counts, which

SUBMITTER: Lindstrom HJG 

PROVIDER: S-EPMC7745411 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature