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The ? subunit and NTPase HelD institute a two-pronged mechanism for RNA polymerase recycling.

ABSTRACT: Cellular RNA polymerases (RNAPs) can become trapped on DNA or RNA, threatening genome stability and limiting free enzyme pools, but how RNAP recycling into active states is achieved remains elusive. In Bacillus subtilis, the RNAP ? subunit and NTPase HelD have been implicated in RNAP recycling. We structurally analyzed Bacillus subtilis RNAP-?-HelD complexes. HelD has two long arms: a Gre cleavage factor-like coiled-coil inserts deep into the RNAP secondary channel, dismantling the active site and displacing RNA, while a unique helical protrusion inserts into the main channel, prying the ? and ?' subunits apart and, aided by ?, dislodging DNA. RNAP is recycled when, after releasing trapped nucleic acids, HelD dissociates from the enzyme in an ATP-dependent manner. HelD abundance during slow growth and a dimeric (RNAP-?-HelD)2 structure that resembles hibernating eukaryotic RNAP I suggest that HelD might also modulate active enzyme pools in response to cellular cues.

SUBMITTER: Pei HH 

PROVIDER: S-EPMC7749165 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature

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The δ subunit and NTPase HelD institute a two-pronged mechanism for RNA polymerase recycling.

Pei Hao-Hong HH   Hilal Tarek T   Chen Zhuo A ZA   Huang Yong-Heng YH   Gao Yuan Y   Said Nelly N   Loll Bernhard B   Rappsilber Juri J   Belogurov Georgiy A GA   Artsimovitch Irina I   Wahl Markus C MC  

Nature communications 20201218 1

Cellular RNA polymerases (RNAPs) can become trapped on DNA or RNA, threatening genome stability and limiting free enzyme pools, but how RNAP recycling into active states is achieved remains elusive. In Bacillus subtilis, the RNAP δ subunit and NTPase HelD have been implicated in RNAP recycling. We structurally analyzed Bacillus subtilis RNAP-δ-HelD complexes. HelD has two long arms: a Gre cleavage factor-like coiled-coil inserts deep into the RNAP secondary channel, dismantling the active site a  ...[more]

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