Ontology highlight
ABSTRACT:
SUBMITTER: Hauk P
PROVIDER: S-EPMC7750011 | biostudies-literature | 2020 Oct
REPOSITORIES: biostudies-literature
Hauk Pricila P Stephens Kristina K Virgile Chelsea C VanArsdale Eric E Pottash Alex Eli AE Schardt John S JS Jay Steven M SM Sintim Herman O HO Bentley William E WE
ACS synthetic biology 20200915 10
We developed a hybrid synthetic circuit that co-opts the genetic regulation of the native bacterial quorum sensing autoinducer-2 and imposes an extra external controller for maintaining tightly controlled gene expression. This dual-input genetic controller was mathematically modeled and, by design, can be operated in three modes: a constitutive mode that enables consistent and high levels of expression; a tightly repressed mode in which there is very little background expression; and an inducibl ...[more]