Dataset Information

A novel angiotensin II peptide vaccine without an adjuvant in mice.

ABSTRACT:

Objectives

We recently developed a novel peptide, AJP001, that possesses both a mouse T-cell epitope and adjuvant action. Direct conjugation to the antigen is useful for peptide vaccines without the addition of adjuvants. In this study, the efficacy of an angiotensin (Ang) II and AJP001-conjugated peptide vaccine (AJ-Ang II) was evaluated in mice.

Methods

The anti-Ang II antibody titer was measured in Balb/C mice following three injections of AJ-Ang II at 2-week intervals. SBP was measured during vaccination of Balb/C mice treated with Ang II infusion (1??g/kg per min).

Results

AJ-Ang II treatment resulted in an increase in the anti-Ang II antibody titer in a dose-dependent manner without the addition of adjuvants. In the analysis of the humoral immune response, AJ-Ang II mainly elicited IgG1 antibodies and IL-4 and IL-10 production, as measured by an enzyme-linked immune absorbent spot assay, which suggests the induction of a Th2 response. Importantly, cotreatment with purified antibodies attenuated Ang II-induced extracellular signal-regulated kinase phosphorylation and nuclear factor (NF)-?B activation in cultured vascular smooth muscle cells. The SBP in immunized mice was significantly lower than that in nonimmunized mice (135.9?±?8.5 vs. 154.9?±?16.8?mmHg, P?=?0.02). Furthermore, Ang II-induced perivascular fibrosis in the heart was significantly attenuated in immunized mice, which also exhibited decreased mRNA expression of collagen I/III and transforming growth factor-?.

Conclusion

AJ-Ang II may be a simple and useful therapeutic peptide vaccine without the addition of any adjuvants.

SUBMITTER: Nakamaru R

PROVIDER: S-EPMC7752219 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature

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Publications

A novel angiotensin II peptide vaccine without an adjuvant in mice.

Nakamaru Ryo R Nakagami Hironori H Hayashi Hiroki H Sun Jiao J Tenma Akiko A Yamamoto Koichi K Shimamura Munehisa M Morishita Ryuichi R Rakugi Hiromi H

Journal of hypertension 20210101 1

<h4>Objectives</h4>We recently developed a novel peptide, AJP001, that possesses both a mouse T-cell epitope and adjuvant action. Direct conjugation to the antigen is useful for peptide vaccines without the addition of adjuvants. In this study, the efficacy of an angiotensin (Ang) II and AJP001-conjugated peptide vaccine (AJ-Ang II) was evaluated in mice.<h4>Methods</h4>The anti-Ang II antibody titer was measured in Balb/C mice following three injections of AJ-Ang II at 2-week intervals. SBP was ...[more]

PMID: 32667158

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Dataset Information

A novel angiotensin II peptide vaccine without an adjuvant in mice.

Objectives

Methods

Results

Conclusion

Publications

A novel angiotensin II peptide vaccine without an adjuvant in mice.

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