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Aptamer-Mediated Reversible Transactivation of Gene Expression by Light.

ABSTRACT: The investigation and manipulation of cellular processes with subcellular resolution requires non-invasive tools with spatiotemporal precision and reversibility. Building on the interaction of the photoreceptor PAL with an RNA aptamer, we describe a variation of the CRISPR/dCAS9 system for light-controlled activation of gene expression. This platform significantly reduces the coding space required for genetic manipulation and provides a strong on-switch with almost no residual activity in the dark. It adds to the current set of modular building blocks for synthetic biological circuit design and is broadly applicable.

SUBMITTER: Renzl C

PROVIDER: S-EPMC7756287 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature

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Aptamer-Mediated Reversible Transactivation of Gene Expression by Light.

Renzl Christian C Kakoti Ankana A Mayer Günter G

Angewandte Chemie (International ed. in English) 20201002 50

The investigation and manipulation of cellular processes with subcellular resolution requires non-invasive tools with spatiotemporal precision and reversibility. Building on the interaction of the photoreceptor PAL with an RNA aptamer, we describe a variation of the CRISPR/dCAS9 system for light-controlled activation of gene expression. This platform significantly reduces the coding space required for genetic manipulation and provides a strong on-switch with almost no residual activity in the da ...[more]

PMID: 32865316

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