Ontology highlight
ABSTRACT:
SUBMITTER: Samaha D
PROVIDER: S-EPMC7756341 | biostudies-literature | 2020 Dec
REPOSITORIES: biostudies-literature
Samaha Doaa D Hamdo Housam H HH Cong Xiaojing X Schumacher Fabian F Banhart Sebastian S Aglar Öznur Ö Möller Heiko M HM Heuer Dagmar D Kleuser Burkhard B Saied Essa M EM Arenz Christoph C
Chemistry (Weinheim an der Bergstrasse, Germany) 20201109 70
Ceramide transfer protein (CERT) mediates non-vesicular transfer of ceramide from endoplasmic reticulum to Golgi apparatus and thus catalyzes the rate-limiting step of sphingomyelin biosynthesis. Usually, CERT ligands are evaluated in tedious binding assays or non-homogenous transfer assays using radiolabeled ceramides. Herein, a facile and sensitive assay for CERT, based on Förster resonance energy transfer (FRET), is presented. To this end, we mixed donor and acceptor vesicles, each containing ...[more]