Dataset Information

Genome-wide dissection of hybridization for fiber quality- and yield-related traits in upland cotton.

ABSTRACT: An evaluation of combining ability can facilitate the selection of suitable parents and superior F₁ hybrids for hybrid cotton breeding, although the molecular genetic basis of combining ability has not been fully characterized. In the present study, 282 female parents were crossed with four male parents in accordance with the North Carolina II mating scheme to generate 1128 hybrids. The parental lines were genotyped based on restriction site-associated DNA sequencing and 306 814 filtered single nucleotide polymorphisms were used for genome-wide association analysis involving the phenotypes, general combining ability (GCA) values, and specific combining ability values of eight fiber quality- and yield-related traits. The main results were: (i) all parents could be clustered into five subgroups based on population structure analyses and the GCA performance of the female parents had significant differences between subgroups; (ii) 20 accessions with a top 5% GCA value for more than one trait were identified as elite parents for hybrid cotton breeding; (iii) 120 significant single nucleotide polymorphisms, clustered into 66 quantitative trait loci, such as the previously reported Gh_A07G1769 and GhHOX3 genes, were found to be significantly associated with GCA; and (iv) identified quantitative trait loci for GCA had a cumulative effect on GCA of the accessions. Overall, our results suggest that pyramiding the favorable loci for GCA may improve the efficiency of hybrid cotton breeding.

SUBMITTER: Geng X

PROVIDER: S-EPMC7756405 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature

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Genome-wide dissection of hybridization for fiber quality- and yield-related traits in upland cotton.

Geng Xiaoli X Sun Gaofei G Qu Yujie Y Sarfraz Zareen Z Jia Yinhua Y He Shoupu S Pan Zhaoe Z Sun Junling J Iqbal Muhammad S MS Wang Qinglian Q Qin Hongde H Liu Jinhai J Liu Hui H Yang Jun J Ma Zhiying Z Xu Dongyong D Yang Jinlong J Zhang Jinbiao J Li Zhikun Z Cai Zhongmin Z Zhang Xuelin X Zhang Xin X Zhou Guanyin G Li Lin L Zhu Haiyong H Wang Liru L Pang Baoyin B Du Xiongming X

The Plant journal : for cell and molecular biology 20201111 5

An evaluation of combining ability can facilitate the selection of suitable parents and superior F<sub>1</sub> hybrids for hybrid cotton breeding, although the molecular genetic basis of combining ability has not been fully characterized. In the present study, 282 female parents were crossed with four male parents in accordance with the North Carolina II mating scheme to generate 1128 hybrids. The parental lines were genotyped based on restriction site-associated DNA sequencing and 306 814 filte ...[more]

PMID: 32996179

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Project description:Early-maturity varieties of upland cotton are becoming increasingly important for farmers to improve their economic benefits through double cropping practices and mechanical harvesting production in China. However, fiber qualities of early-maturing varieties are relatively poor compared with those of middle- and late- maturing ones. Therefore, it is crucial for researchers to elucidate the genetic bases controlling fiber-quality related traits in early-maturity cultivars, and to improve synergistically cotton earliness and fiber quality. Here, multi-locus genome-wide association studies (ML-GWAS) were conducted in a panel consisting of 160 early-maturing cotton accessions. Each accession was genotyped by 72,792 high-quality single nucleotide polymorphisms (SNPs) using specific-locus amplified fragment sequencing (SLAF-seq) approach, and fiber quality-related traits under four environmental conditions were measured. Applying at least three ML-GWAS methods, a total of 70 significant quantitative trait nucleotides (QTNs) were identified to be associated with five objective traits, including fiber length (FL), fiber strength (FS), fiber micronaire (FM), fiber uniformity (FU) and fiber elongation (FE). Among these QTNs, D11_21619830, A05_28352019 and D03_34920546 were found to be significantly associated with FL, FS, and FM, respectively, across at least two environments. Among 96 genes located in the three target genomic regions (A05: 27.95 28.75, D03: 34.52 35.32, and D11: 21.22 22.02 Mbp), six genes (Gh_A05G2325, Gh_A05G2329, Gh_A05G2334, Gh_D11G1853, Gh_D11G1876, and Gh_D11G1879) were detected to be highly expressed in fibers relative to other eight tissues by transcriptome sequencing method in 12 cotton tissues. Together, multiple favorable QTN alleles and six candidate key genes were characterized to regulate fiber development in early-maturity cotton. This will lay a solid foundation for breeding novel cotton varieties with earliness and excellent fiber-quality in the future.

Dataset Information

Genome-wide dissection of hybridization for fiber quality- and yield-related traits in upland cotton.

Publications

Genome-wide dissection of hybridization for fiber quality- and yield-related traits in upland cotton.

Similar Datasets

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