Would 20 nm Filtered Fetal Bovine Serum-Supplemented Media Support Growth of CHO and HEK-293 Cells?
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ABSTRACT:
SUBMITTER: Manukyan L
PROVIDER: S-EPMC7756488 | biostudies-literature | 2020 Dec
REPOSITORIES: biostudies-literature
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Would 20 nm Filtered Fetal Bovine Serum-Supplemented Media Support Growth of CHO and HEK-293 Cells?
Manukyan Levon L Marinaki Maria-Eleni ME Mihranyan Albert A
ACS applied bio materials 20201208 12
Virus safety of fetal bovine serum (FBS) is a critical issue for cell culture and clinical applications of cell therapies. The size exclusion filtration of FBS-supplemented cell culture media through small-size virus retentive filter paper is presented to investigate its effect on cell culture. A substantial proportion of proteins (ca. 45%) was removed by nanofiltration, yet important transport proteins (albumin, fetuins, macroglobulins, transferrin) were unaffected. The cell viability of Chines ...[more]
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