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Strategies for ¹ H-Detected Dynamic Nuclear Polarization Magic-Angle Spinning NMR Spectroscopy.

ABSTRACT: Combining dynamic nuclear polarization with proton detection significantly enhances the sensitivity of magic-angle spinning NMR spectroscopy. Herein, the feasibility of proton-detected experiments with slow (10?kHz) magic angle spinning was demonstrated. The improvement in sensitivity permits the acquisition of indirectly detected ¹⁴ N?NMR spectra allowing biomolecular structures to be characterized without recourse to isotope labelling. This provides a new tool for the structural characterization of environmental and medical samples, in which isotope labelling is frequently intractable.

SUBMITTER: Concistre M

PROVIDER: S-EPMC7756879 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature

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Strategies for 1 H-Detected Dynamic Nuclear Polarization Magic-Angle Spinning NMR Spectroscopy.

Concistré Maria M Paul Subhradip S Carravetta Marina M Kuprov Ilya I Williamson Philip T F PTF

Chemistry (Weinheim an der Bergstrasse, Germany) 20201103 68

Combining dynamic nuclear polarization with proton detection significantly enhances the sensitivity of magic-angle spinning NMR spectroscopy. Herein, the feasibility of proton-detected experiments with slow (10 kHz) magic angle spinning was demonstrated. The improvement in sensitivity permits the acquisition of indirectly detected 14 N NMR spectra allowing biomolecular structures to be characterized without recourse to isotope labelling. This provides a new tool for the structural cha ...[more]

PMID: 32827182

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