Dual RNA-Sequencing of Mycobacterium tuberculosis-Infected Cells from a Murine Infection Model.
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ABSTRACT:
SUBMITTER: Pisu D
PROVIDER: S-EPMC7756974 | biostudies-literature | 2020 Dec
REPOSITORIES: biostudies-literature
Publications
Dual RNA-Sequencing of <i>Mycobacterium tuberculosis</i>-Infected Cells from a Murine Infection Model.
Pisu Davide D Huang Lu L Rin Lee Bom Nae BN Grenier Jennifer K JK Russell David G DG
STAR protocols 20201006 3
Dual RNA-sequencing is a powerful technique to assess both bacterial and host transcriptomes in an unbiased way. We developed a protocol to perform Dual RNA-seq on <i>in vivo</i>-derived macrophage populations infected with <i>Mycobacterium tuberculosis</i>. Here, we provide a practical step-by-step guide to execute the protocol on Mtb-infected cells from a murine infection model. Our protocol can also be easily applied to perform Dual RNA-seq on <i>in vitro</i>-derived cells as well as differen ...[more]
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