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Protocol for Patch-Seq of Small Interneurons.

ABSTRACT: Obtaining electrophysiological recordings and gene expression information from the same neuron (Patch-seq) brings forward a unique opportunity to study the transcriptional correlates of functional properties and vice versa. Here, we provide a detailed Patch-seq protocol tailored to the specialized demands of studying small interneurons. Focusing on the technically demanding process of transitioning between patch recordings and cell extraction, our protocol describes and troubleshoots steps for successfully collecting small interneurons, allowing for multi-modal Patch-seq interrogation of this crucial cell type.

SUBMITTER: Lipovsek M

PROVIDER: S-EPMC7757288 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature

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Protocol for Patch-Seq of Small Interneurons.

Lipovsek Marcela M Browne Lorcan L Grubb Matthew S MS

STAR protocols 20201022 3

Obtaining electrophysiological recordings and gene expression information from the same neuron (Patch-seq) brings forward a unique opportunity to study the transcriptional correlates of functional properties and vice versa. Here, we provide a detailed Patch-seq protocol tailored to the specialized demands of studying small interneurons. Focusing on the technically demanding process of transitioning between patch recordings and cell extraction, our protocol describes and troubleshoots steps for s ...[more]

PMID: 33377040

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