Ontology highlight
ABSTRACT:
SUBMITTER: Nakahara TS
PROVIDER: S-EPMC7757322 | biostudies-literature | 2020 Dec
REPOSITORIES: biostudies-literature
Nakahara Thiago Seike TS Carvalho Vinicius Miessler de Andrade VMA Souza Mateus Augusto de Andrade MAA Trintinalia Guilherme Ziegler GZ Papes Fabio F
STAR protocols 20201029 3
This protocol combines fluorescent <i>in situ</i> hybridization and immunostaining to simultaneously detect, in histological sections from the same animal, subpopulations of neurons activated after two episodes of sensory stimulation. It allows the identification of groups of cells singly activated by either stimulus or co-activated by both stimuli. Our method results in nuclear staining for <i>c-Fos</i> mRNA and c-Fos protein, allowing better spatial and temporal resolution than previously publ ...[more]