Detecting CO2-Sensitive Hemichannels in Neurons in Acute Brain Slices.
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ABSTRACT:
SUBMITTER: Hill E
PROVIDER: S-EPMC7757330 | biostudies-literature | 2020 Dec
REPOSITORIES: biostudies-literature
Publications
Detecting CO<sub>2</sub>-Sensitive Hemichannels in Neurons in Acute Brain Slices.
Hill Emily E Dale Nicholas N Wall Mark J MJ
STAR protocols 20201023 3
This protocol provides two independent methods to functionally detect the neuronal expression of CO<sub>2</sub>-sensitive hemichannels. These hemichannels (consisting of connexins 26 or 30) are directly gated by CO<sub>2</sub>, independent of pH changes and until recently were thought to be only expressed by glia. This protocol outlines a method to change the concentration of CO<sub>2</sub> without changing pH, using isohydric solutions and then utilizing this to detect opening and closing of fu ...[more]
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