Replication-Independent Generation and Morphological Analysis of Flavivirus Replication Organelles.
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ABSTRACT:
SUBMITTER: Goellner S
PROVIDER: S-EPMC7757399 | biostudies-literature | 2020 Dec
REPOSITORIES: biostudies-literature
Publications
Replication-Independent Generation and Morphological Analysis of Flavivirus Replication Organelles.
Goellner Sarah S Cerikan Berati B Cortese Mirko M Neufeldt Christopher J CJ Haselmann Uta U Bartenschlager Ralf R
STAR protocols 20201125 3
Positive-strand RNA viruses replicate in distinct membranous structures called replication organelles (ROs). Mechanistic studies of RO formation have been difficult because perturbations affecting viral replication have an impact on viral protein amounts, thus affecting RO biogenesis. Here, we present a detailed guide on how to use a replication-independent expression system, designated pIRO (plasmid-induced replication organelle formation), inducing bona fide flavivirus ROs in transfected cells ...[more]
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