Protocol to Reprogram Human Menstrual Blood-Derived Stromal Cells to Generate AOX15-iPSCs.
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ABSTRACT:
SUBMITTER: Sanzhez-Mata A
PROVIDER: S-EPMC7757400 | biostudies-literature | 2020 Dec
REPOSITORIES: biostudies-literature
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Protocol to Reprogram Human Menstrual Blood-Derived Stromal Cells to Generate AOX15-iPSCs.
Sanzhez-Mata Alicia A Ferez-Gomez Alberto A Gonzalez-Muñoz Elena E
STAR protocols 20201125 3
Cell reprogramming has revolutionized the fields of cell and regenerative biology. However, human induced pluripotent stem cell (iPSC) derivation remains inefficient and variable. Here, we present a protocol that uses human menstrual blood-derived stromal cells (MnSCs), which are susceptible to reprogramming, as a source of somatic cells. We describe an oocyte-based reprogramming combination to generate AOX15-iPSCs that can be used to study different states of pluripotency. For complete details ...[more]
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