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A Quantitative Imaging-Based Protocol for Yeast Growth and Survival on Agar Plates.

ABSTRACT: We present a detailed protocol that describes the evaluation of the growth and survival of yeast cells by quantitatively analyzing spotting assays. This simple method reproducibly detects and quantifies subtle differences in growth by measuring the density of cells within a single spot of defined size on an image of a spotting assay. Our protocol is tailored specifically for low-throughput applications, can be easily adapted for specific experimental conditions, and is accessible to yeast experts and non-experts alike. For an example of the execution of this protocol, please refer to DiGregorio et al. (Di Gregorio et al., 2020).

SUBMITTER: Petropavlovskiy AA

PROVIDER: S-EPMC7757406 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature

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A Quantitative Imaging-Based Protocol for Yeast Growth and Survival on Agar Plates.

Petropavlovskiy Andrey A AA Tauro Michael G MG Lajoie Patrick P Duennwald Martin L ML

STAR protocols 20201125 3

We present a detailed protocol that describes the evaluation of the growth and survival of yeast cells by quantitatively analyzing spotting assays. This simple method reproducibly detects and quantifies subtle differences in growth by measuring the density of cells within a single spot of defined size on an image of a spotting assay. Our protocol is tailored specifically for low-throughput applications, can be easily adapted for specific experimental conditions, and is accessible to yeast expert ...[more]

PMID: 33377076

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