Differential Centrifugation to Enrich Bacterial Ribonucleoprotein Bodies (BR bodies) from Caulobacter crescentus.
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ABSTRACT:
SUBMITTER: Muthunayake NS
PROVIDER: S-EPMC7757671 | biostudies-literature | 2020 Dec
REPOSITORIES: biostudies-literature
Publications
Differential Centrifugation to Enrich Bacterial Ribonucleoprotein Bodies (BR bodies) from <i>Caulobacter crescentus</i>.
Muthunayake Nisansala S NS Al-Husini Nadra N Schrader Jared M JM
STAR protocols 20201209 3
Bacterial RNP bodies (BR bodies) contain the mRNA decay machinery, but the collection of associated RNAs and proteins are poorly defined. Here, we present a protocol for the rapid differential centrifugation-based enrichment of BR bodies from <i>Caulobacter crescentus</i> cells. As native BR bodies are highly labile and dissociate by degrading internal mRNAs, an active site mutant of RNase E, which blocks dissolution of BR bodies, allows BR-body stabilization during enrichment. For complete deta ...[more]
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