Protocol for brain-wide or region-specific microglia depletion and repopulation in adult mice.
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ABSTRACT:
SUBMITTER: Willis EF
PROVIDER: S-EPMC7757678 | biostudies-literature | 2020 Dec
REPOSITORIES: biostudies-literature
Publications
Protocol for brain-wide or region-specific microglia depletion and repopulation in adult mice.
Willis Emily F EF Vukovic Jana J
STAR protocols 20201213 3
The advent of tools enabling the direct manipulation of microglia has furthered our understanding of their role in health and disease. Here, we present a detailed protocol allowing for microglia turnover in adult CX<sub>3</sub>CR<sub>1</sub><sup>creERT2</sup> × iDTR or CX<sub>3</sub>CR<sub>1</sub><sup>creERT2</sup> × iDTR × tdTomato<sup>flox</sup> mice, either in a brain-wide or region-specific manner, and their subsequent isolation for downstream applications. This protocol may be used to explo ...[more]
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