Unknown

Dataset Information

0

Two-parameter single-molecule analysis for measurement of chromatin mobility.


ABSTRACT: This protocol provides a two-parameter analysis of single-molecule tracking (SMT) trajectories of Halo-tagged histones in living adherent cell lines and unveils a chromatin mobility landscape composed of five chromatin types, ranging from low to high mobility. When the analysis is applied to Halo-tagged, chromatin-binding proteins, it associates chromatin interaction properties with known functions in a way that previously used SMT parameters did not. For complete information on the use and execution of this protocol, please refer to Lerner et al. (2020).

SUBMITTER: Lerner J 

PROVIDER: S-EPMC7757679 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC7483934 | biostudies-literature
| S-EPMC8096815 | biostudies-literature
| S-EPMC8113488 | biostudies-literature
| S-EPMC8287188 | biostudies-literature
| S-EPMC4823137 | biostudies-literature
| S-EPMC8080847 | biostudies-literature
| S-EPMC7001875 | biostudies-literature
| S-EPMC7980255 | biostudies-literature
| S-EPMC6326756 | biostudies-literature
| S-EPMC5679410 | biostudies-literature