Ontology highlight
ABSTRACT:
SUBMITTER: Li J
PROVIDER: S-EPMC7757697 | biostudies-literature | 2020 Dec
REPOSITORIES: biostudies-literature
Li Jixue J Li Yanjie Y Pawlik Kevin M KM Napierala Jill S JS Napierala Marek M
The CRISPR journal 20201103 6
We describe a protocol for the precise integration of exogenous DNA into user-defined genomic loci in cultured cells. This strategy first introduces a promoter and a <i>lox</i> site to a specific location via a Cas9-induced double-strand break. Second, a gene of interest (GOI) is inserted into the <i>lox</i> site via Cre-<i>lox</i> recombination. Upon correct insertion, a <i>cis</i>-linked antibiotic resistance gene will be expressed from a promoter introduced into the genome in the first step a ...[more]