Comparable V?2 Cell Functional Characteristics in Virally Suppressed People Living with HIV and Uninfected Individuals.

ABSTRACT: Crosstalk between innate and adaptive pathways is a critical component to developing an effective, lasting immune response. Among natural effector cells, innate-like ?? T cells promote immunity by facilitating communication between the two compartments and exerting cytotoxic effector functions. Dysregulation of ?? T cell populations is a byproduct of primary Humanimmunodeficiency virus (HIV) infection. This is most pronounced in the depletion and loss of function within cells expressing a V?9V?2 TCR (V?2 cells). Whether or not prolonged viral suppression mediated by antiretroviral therapy (ART) can reverse these effects has yet to be determined. In this study, we present evidence of similar V?2 cell functional responses within a cohort of people living with HIV (PLWH) that has been stably suppressed for >1 year and uninfected donors. Through the use of aminobisphosphonate drugs, we were able to generate a comprehensive comparison between ex vivo and expanded V?2 cells within each group. Both groups had largely similar compositions of memory and effector phenotypes, post-expansion TCR repertoire diversity, and cytotoxic capabilities. Our findings support the notion that ART promotes the recovery of V?2 polyfunctionality and provides insight for strategies aiming to reconstitute the full immune response after infection with HIV.

SUBMITTER: Clohosey ML 

PROVIDER: S-EPMC7760715 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature