Ontology highlight
ABSTRACT:
SUBMITTER: Ribeiro Reily Rocha C
PROVIDER: S-EPMC7760831 | biostudies-literature | 2020 Dec
REPOSITORIES: biostudies-literature
Rocha Clarissa Ribeiro Reily CRR Reily Rocha Alexandre A Molina Silva Matheus M Rodrigues Gomes Luciana L Teatin Latancia Marcela M Andrade Tomaz Marina M de Souza Izadora I Karolynne Seregni Monteiro Linda L Menck Carlos Frederico Martins CFM
Cells 20201201 12
Glioblastoma is a severe type of brain tumor with a poor prognosis and few therapy options. Temozolomide (TMZ) is one of these options, however, with limited success, and failure is mainly due to tumor resistance. In this work, genome-wide CRISPR-Cas9 lentiviral screen libraries for gene knockout or activation were transduced in the human glioblastoma cell line, aiming to identify genes that modulate TMZ resistance. The sgRNAs enriched in both libraries in surviving cells after TMZ treatment wer ...[more]