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Three Novel Clostridia Isolates Produce n-Caproate and iso-Butyrate from Lactate: Comparative Genomics of Chain-Elongating Bacteria.


ABSTRACT: The platform chemicals n-caproate and iso-butyrate can be produced by anaerobic fermentation from agro-industrial residues in a process known as microbial chain elongation. Few lactate-consuming chain-elongating species have been isolated and knowledge on their shared genetic features is still limited. Recently we isolated three novel clostridial strains (BL-3, BL-4, and BL-6) that convert lactate to n-caproate and iso-butyrate. Here, we analyzed the genetic background of lactate-based chain elongation in these isolates and other chain-elongating species by comparative genomics. The three strains produced n-caproate, n-butyrate, iso-butyrate, and acetate from lactate, with the highest proportions of n-caproate (18%) for BL-6 and of iso-butyrate (23%) for BL-4 in batch cultivation at pH 5.5. They show high genomic heterogeneity and a relatively small core-genome size. The genomes contain highly conserved genes involved in lactate oxidation, reverse ?-oxidation, hydrogen formation and either of two types of energy conservation systems (Rnf and Ech). Including genomes of another eleven experimentally validated chain-elongating strains, we found that the chain elongation-specific core-genome encodes the pathways for reverse ?-oxidation, hydrogen formation and energy conservation, while displaying substantial genome heterogeneity. Metabolic features of these isolates are important for biotechnological applications in n-caproate and iso-butyrate production.

SUBMITTER: Liu B 

PROVIDER: S-EPMC7764203 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature

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Three Novel <i>Clostridia</i> Isolates Produce <i>n</i>-Caproate and <i>iso</i>-Butyrate from Lactate: Comparative Genomics of Chain-Elongating Bacteria.

Liu Bin B   Popp Denny D   Müller Nicolai N   Sträuber Heike H   Harms Hauke H   Kleinsteuber Sabine S  

Microorganisms 20201211 12


The platform chemicals <i>n</i>-caproate and <i>iso</i>-butyrate can be produced by anaerobic fermentation from agro-industrial residues in a process known as microbial chain elongation. Few lactate-consuming chain-elongating species have been isolated and knowledge on their shared genetic features is still limited. Recently we isolated three novel clostridial strains (BL-3, BL-4, and BL-6) that convert lactate to <i>n</i>-caproate and <i>iso</i>-butyrate. Here, we analyzed the genetic backgroun  ...[more]

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