Comparative Determination of Cytotoxicity of Sub-10 nm Copper Nanoparticles to Prokaryotic and Eukaryotic Systems.

ABSTRACT: Copper nanoparticles demonstrate antibacterial activity, but their toxicity to eukaryotic systems is less understood. Here, we carried out a comparative study to determine the biocompatibility and cytotoxicity of sub-10 nm copper nanoparticles to a variety of biological systems, including prokaryotic cells (Escherichia coli), yeast, mammalian cell lines (HEK293T, PC12), and zebrafish embryos. We determined the bearing threshold for the cell-death-inducing concentration of copper nanoparticles by probing cell growth, viability, as well as embryological features. To exclude the partial toxicity effect from the remnant reactants, we developed a purification approach using agarose gel electrophoresis. Purified CuONP solution inhibits bacterial growth and causes eukaryotic cell death at

SUBMITTER: Skeeters SS 

PROVIDER: S-EPMC7764564 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature