Dataset Information

Recyclable Multilayer Packaging by Means of Thermoreversibly Crosslinking Adhesive in the Context of Food Law.

ABSTRACT: Lacking recyclability of multilayer packaging can be overcome by using a thermoreversible crosslinking adhesive consisting of maleimide- and furan-functionalized polyurethane-(PU-)prepolymers, reacting in a Diels-Alder-reaction. Here, the furan-functionalized PU-prepolymer carries furan-side-chains to avoid the usage of an additional crosslinking agent. Thus, N?(2?hydroxyethyl)maleimide and furfurylamine are the only two chemicals contained in the adhesive that are not listed in the appendix of EU Regulation 10/2011. Using migration modelling, it could be shown that, at 23 °C, both chemicals have lag-times of only a few minutes if 45 µm PE is used as a barrier. However, if the residual content is below 30 mg/kg, the legally specified maximum amount of 0.01 mg/kg food is not reached. After determining the diffusion coefficients and the activation energy of diffusion through ethylene-vinyl alcohol copolymer (EVOH), it could be determined that the lag-time of the migrants can be extended to at least 9 years by the use of 3 µm EVOH. From a food law point of view, the use of the described adhesive is possible if the above?mentioned measures are complied.

SUBMITTER: Kaiser KMA

PROVIDER: S-EPMC7765215 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature

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Recyclable Multilayer Packaging by Means of Thermoreversibly Crosslinking Adhesive in the Context of Food Law.

Kaiser Katharina M A KMA Ewender Johann J Welle Frank F

Polymers 20201215 12

Lacking recyclability of multilayer packaging can be overcome by using a thermoreversible crosslinking adhesive consisting of maleimide- and furan-functionalized polyurethane-(PU-)prepolymers, reacting in a Diels-Alder-reaction. Here, the furan-functionalized PU-prepolymer carries furan-side-chains to avoid the usage of an additional crosslinking agent. Thus, <i>N</i>‑(2‑hydroxyethyl)maleimide and furfurylamine are the only two chemicals contained in the adhesive that are not listed in the appen ...[more]

PMID: 33333812

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Project description:Viruses assemble large macromolecular repeat structures that become part of the infectious particles or virions. Ribonucleocapsids (RNCs) of negative strand RNA viruses are a prime example where repetition of nucleoprotein (NP) along the genome creates a core polymeric helical scaffold that accommodates other nucleocapsid proteins including viral polymerase. The RNCs are transported through the cytosol for packaging into virions through association with viral matrix proteins at cell membranes. We hypothesized that RNC would be ideal targets for crosslinkers engineered to promote aberrant protein-protein interactions, thereby blocking their orderly transport and packaging. Previously, we had generated single-domain antibodies (sdAbs) against Filoviruses that have all targeted highly conserved C-terminal regions of NP known to be repetitively exposed along the length of the RNCs of Marburgvirus (MARV) and Ebolavirus (EBOV). Our crosslinker design consisted of dimeric sdAb expressed intracellularly, which we call Xintrabodies (X- for crosslinking). Electron microscopy of purified NP polymers incubated with purified sdAb constructs showed NP aggregation occurred in a genus-specific manner with dimeric and not monomeric sdAb. A virus-like particle (VLP) assay was used for initial evaluation where we found that dimeric sdAb inhibited NP incorporation into VP40-based VLPs whereas monomeric sdAb did not. Inhibition of NP packaging was genus specific. Confocal microscopy revealed dimeric sdAb was diffuse when expressed alone but focused on pools of NP when the two were coexpressed, while monomeric sdAb showed ambivalent partition. Infection of stable Vero cell lines expressing dimeric sdAb specific for either MARV or EBOV NP resulted in smaller plaques and reduced progeny of cognate virus relative to wild-type Vero cells. Though the impact was marginal at later time-points, the collective data suggest that viral replication can be reduced by crosslinking intracellular NP using relatively small amounts of dimeric sdAb to restrict NP packaging. The stoichiometry and ease of application of the approach would likely benefit from transitioning away from intracellular expression of crosslinking sdAb to exogenous delivery of antibody. By retuning sdAb specificity, the approach of crosslinking highly conserved regions of assembly critical proteins may well be applicable to inhibiting replication processes of a broad spectrum of viruses.

Dataset Information

Recyclable Multilayer Packaging by Means of Thermoreversibly Crosslinking Adhesive in the Context of Food Law.

Publications

Recyclable Multilayer Packaging by Means of Thermoreversibly Crosslinking Adhesive in the Context of Food Law.

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