Ontology highlight
ABSTRACT:
SUBMITTER: Ilina TV
PROVIDER: S-EPMC7765405 | biostudies-literature | 2020 Dec
REPOSITORIES: biostudies-literature
Ilina Tatiana V TV Xi Zhaoyong Z Brosenitsch Teresa T Sluis-Cremer Nicolas N Ishima Rieko R
International journal of molecular sciences 20201215 24
NMR studies of large proteins, over 100 kDa, in solution are technically challenging and, therefore, of considerable interest in the biophysics field. The challenge arises because the molecular tumbling of a protein in solution considerably slows as molecular mass increases, reducing the ability to detect resonances. In fact, the typical <sup>1</sup>H-<sup>13</sup>C or <sup>1</sup>H-<sup>15</sup>N correlation spectrum of a large protein, using a <sup>13</sup>C- or <sup>15</sup>N-uniformly labele ...[more]