Ontology highlight
ABSTRACT:
SUBMITTER: Zazeri G
PROVIDER: S-EPMC7766160 | biostudies-literature | 2020 Dec
REPOSITORIES: biostudies-literature
Zazeri Gabriel G Povinelli Ana Paula Ribeiro APR Lima Marcelo de Freitas MF Cornélio Marinônio Lopes ML
Biomedicines 20201218 12
In this work, for the first time, details of the complex formed by heat shock protein 70 (HSP70) independent nucleotide binding domain (NBD) and piperine were characterized through experimental and computational molecular biophysical methods. Fluorescence spectroscopy results revealed positive cooperativity between the two binding sites. Circular dichroism identified secondary conformational changes. Molecular dynamics along with molecular mechanics Poisson Boltzmann surface area (MM/PBSA) reinf ...[more]