Unknown

Dataset Information

0

HDL-Mediated Cholesterol Efflux and Plasma Loading Capacities Are Altered in Subjects with Metabolically- but Not Genetically Driven Non-Alcoholic Fatty Liver Disease (NAFLD).


ABSTRACT: Background. Non-alcoholic fatty liver disease (NAFLD) increases the risk of atherosclerosis but this risk may differ between metabolically- vs. genetically-driven NAFLD. High-density lipoprotein (HDL)-mediated cholesterol efflux (CEC) and plasma loading capacity (CLC) are key factors in atherogenesis. Aims. To test whether CEC and CLC differ between metabolically- vs. genetically-determined NAFLD. Methods: CEC and CLC were measured in 19 patients with metabolic NAFLD and wild-type PNPLA3 genotype (Group M), 10 patients with genetic NAFLD carrying M148M PNPLA3 genotype (Group G), and 10 controls PNPLA3 wild-types and without NAFLD. CEC and CLC were measured ex vivo by isotopic and fluorimetric techniques using cellular models. Results: Compared with Group G, Group M showed reduced total CEC (-18.6%; p < 0.001) as well as that mediated by cholesterol transporters (-25.3% ABCA1; -16.3% ABCG1; -14.8% aqueous diffusion; all p < 0.04). No difference in CEC was found between Group G and controls. The presence of metabolic syndrome further impaired ABCG1-mediated CEC in Group M. Group M had higher plasma-induced CLC than Group G and controls (p < 0.001). Conclusions: Metabolically-, but not genetically-, driven NAFLD associates with dysfunctional HDL-meditated CEC and abnormal CLC. These data suggest that the mechanisms of anti-atherogenic protection in metabolic NAFLD are impaired.

SUBMITTER: Di Costanzo A 

PROVIDER: S-EPMC7766839 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature

altmetric image

Publications

HDL-Mediated Cholesterol Efflux and Plasma Loading Capacities Are Altered in Subjects with Metabolically- but Not Genetically Driven Non-Alcoholic Fatty Liver Disease (NAFLD).

Di Costanzo Alessia A   Ronca Annalisa A   D'Erasmo Laura L   Manfredini Matteo M   Baratta Francesco F   Pastori Daniele D   Di Martino Michele M   Ceci Fabrizio F   Angelico Francesco F   Del Ben Maria M   Pavanello Chiara C   Turri Marta M   Calabresi Laura L   Favari Elda E   Arca Marcello M  

Biomedicines 20201218 12


<b>Background</b>. Non-alcoholic fatty liver disease (NAFLD) increases the risk of atherosclerosis but this risk may differ between metabolically- vs. genetically-driven NAFLD. High-density lipoprotein (HDL)-mediated cholesterol efflux (CEC) and plasma loading capacity (CLC) are key factors in atherogenesis. <b>Aims</b>. To test whether CEC and CLC differ between metabolically- vs. genetically-determined NAFLD. <b>Methods</b>: CEC and CLC were measured in 19 patients with metabolic NAFLD and wil  ...[more]

Similar Datasets

| S-EPMC9387699 | biostudies-literature
| S-EPMC4308988 | biostudies-literature
| S-EPMC3697153 | biostudies-literature
| S-EPMC7176342 | biostudies-literature
| S-EPMC6076293 | biostudies-literature
| S-EPMC3540842 | biostudies-other
| S-EPMC4513993 | biostudies-literature
| S-EPMC4731105 | biostudies-literature
| S-EPMC3329398 | biostudies-literature
| S-EPMC5328158 | biostudies-literature