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Carnosic acid improves porcine early embryonic development by inhibiting the accumulation of reactive oxygen species.

ABSTRACT: Carnosic acid (CA), a natural catechol rosin diterpene, is used as an additive in animal feeds and human foods. However, the effects of CA on mammalian reproductive processes, especially early embryonic development, are unclear. In this study, we added CA to parthenogenetically activated porcine embryos in an in vitro culture medium to explore the influence of CA on apoptosis, proliferation, blastocyst formation, reactive oxygen species (ROS) levels, glutathione (GSH) levels, mitochondrial membrane potential, and embryonic development-related gene expression. The results showed that supplementation with 10 ?M CA during in vitro culture significantly improved the cleavage rates, blastocyst formation rates, hatching rates, and total numbers of cells of parthenogenetically activated porcine embryos compared with no supplementation. More importantly, supplementation with CA also improved GSH levels and mitochondrial membrane potential, reduced natural ROS levels in blastomeres, upregulated Nanog, Sox2, Gata4, Cox2, Itga5, and Rictor expression, and downregulated Birc5 and Caspase3 expression. These results suggest that CA can improve early porcine embryonic development by regulating oxidative stress. This study elucidates the effects of CA on early embryonic development and their potential mechanisms, and provides new applications for improving the quality of in vitro-developed embryos.

SUBMITTER: Peng YX

PROVIDER: S-EPMC7768177 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature

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Carnosic acid improves porcine early embryonic development by inhibiting the accumulation of reactive oxygen species.

Peng Yan-Xia YX Chen Cheng-Zhen CZ Luo Dan D Yu Wen-Jie WJ Li Sheng-Peng SP Xiao Yue Y Yuan Bao B Liang Shuang S Yao Xue-Rui XR Kim Nam-Hyung NH Jiang Hao H Zhang Jia-Bao JB

The Journal of reproduction and development 20201014 6

Carnosic acid (CA), a natural catechol rosin diterpene, is used as an additive in animal feeds and human foods. However, the effects of CA on mammalian reproductive processes, especially early embryonic development, are unclear. In this study, we added CA to parthenogenetically activated porcine embryos in an in vitro culture medium to explore the influence of CA on apoptosis, proliferation, blastocyst formation, reactive oxygen species (ROS) levels, glutathione (GSH) levels, mitochondrial membr ...[more]

PMID: 33055461

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Carnosic acid improves porcine early embryonic development by inhibiting the accumulation of reactive oxygen species.

Publications

Carnosic acid improves porcine early embryonic development by inhibiting the accumulation of reactive oxygen species.

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